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For the Hsp70 and Hsp90 activity inhibition experiments, Neuro2a cells have been cultured in medium supplemented with 20 µM Ver155008 (Sigma) for 18 hours (h) or 800 µM novobiocin (Sigma) for six h. To dam the proteolytic exercise of 26S proteasome complex, cells had been burdened in medium supplemented with 5 µM MG132 (Sigma) for 18 h. To induce oxidative stress, cells had been handled with 500 µM sodium arsenite (Sigma) for 1 h. To induce ER stress, cells were treated with 5 … Read the rest